1. Publishing
    2. Series
    3. Overview
    4. MAK Collection
    5. The MAK Collection for Occupational Health and Safety
    6. Article
    7. Volume 9 (2024)
    8. Issue 2
    9. Benzoesäure – Bestimmung von Benzoesäure in der Luft am Arbeitsplatz mittels Hochleistungsflüssigkeitschromatographie (HPLC-DAD)
Cover: The MAK Collection for Occupational Health and Safety

The MAK Collection for Occupational Health and Safety

German Research Foundation – Permanent Senate Commission for the Investigation of Health Hazards of Chemical Compounds in the Work Area
(MAK Commission)

ISSN 2509-2383



Benzoesäure – Bestimmung von Benzoesäure in der Luft am Arbeitsplatz mittels Hochleistungsflüssigkeitschromatographie (HPLC-DAD)

Luftanalysen-Methode

George Dragan1 (Methodenentwicklung)
  Ralph Hebisch1 (Leitung der Arbeitsgruppe „Luftanalysen“ der Ständigen Senatskommission zur Prüfung gesundheitsschädlicher Arbeitsstoffe, Deutsche Forschungsgemeinschaft)
Adela Frenzen2 (Methodenprüfung)
Lutz Nitschke2 (Methodenprüfung)
  Andrea Hartwig3 (Vorsitz der Ständigen Senatskommission zur Prüfung gesundheitsschädlicher Arbeitsstoffe, Deutsche Forschungsgemeinschaft)
  MAK Commission4

1 Bundesanstalt für Arbeitsschutz und Arbeitsmedizin (BAuA), Friedrich-Henkel-Weg 1–25, 44149 Dortmund, Deutschland
2 Bayerische Landesamt für Gesundheit und Lebensmittelsicherheit (LGL), Pfarrstraße 3, 80538 München, Deutschland
3 Institut für Angewandte Biowissenschaften, Abteilung Lebensmittelchemie und Toxikologie, Karlsruher Institut für Technologie (KIT), Adenauerring 20a, Geb. 50.41, 76131 Karlsruhe, Deutschland
4 Ständige Senatskommission zur Prüfung gesundheitsschädlicher Arbeitsstoffe, Deutsche Forschungsgemeinschaft, Kennedyallee 40, 53175 Bonn, Deutschland

Abstract

The working group “Air Analyses” of the German Senate Commission for the Investigation of Health Hazards of Chemical Compounds in the Work Area (MAK Commission) developed and verified the presented analytical method. This analytical method permits the determination of benzoic acid [65-85-0] occurring as inhalable particles and vapour in workplace air. The concentration range covers one tenth up to twice the currently valid Occupational Exposure Limit Value (OELV) in Germany of 0.5 mg/m3. Sampling is performed using a flow-regulated pump to draw a defined volume of air through a binder-free quartz fibre filter followed by a sorbent tube filled with silica gel. The volumetric flow rate is 1 l/min and the sampling duration is 2 hours (which corresponds to a sampling volume of 120 l). The collected benzoic acid is extracted with methanol and subsequently analysed by means of high-performance liquid chromatography using diode array detection. Quantitative determination is based on a multiple-point calibration function. For an air sample volume of 120 litres, the relative limit of quantification (LOQ) is 0.0023 mg/m3. The mean recovery is 96.3% and the expanded uncertainty for the validation range of 0.05 to 1 mg/m3 is 18%.

Keywords

benzoic acid, air analyses, analytical method, workplace measurement, hazardous substance, high-performance liquid chromatography, diode array detection, HPLC-DAD, quartz fibre filter, silica gel