Quecksilber und Quecksilberverbindungen – Bestimmung von Quecksilber in Blut und Urin mittels Kaltdampf‐AAS
Biomonitoring-Methode
Peter Heitland1Thomas Göen2
Andrea Hartwig3
MAK Commission4
1 MVZ Medizinisches Labor Bremen GmbH, Haferwende 12, 28357 Bremen, Deutschland
2 Friedrich-Alexander-Universität Erlangen-Nürnberg, Institut und Poliklinik für Arbeits-, Sozial- und Umweltmedizin, Henkestraße 9–11, 91054 Erlangen, Deutschland
3 Institut für Angewandte Biowissenschaften, Abteilung Lebensmittelchemie und Toxikologie, Karlsruher Institut für Technologie (KIT), Adenauerring 20a, Geb. 50.41, 76131 Karlsruhe, Deutschland
4 Ständige Senatskommission zur Prüfung gesundheitsschädlicher Arbeitsstoffe, Deutsche Forschungsgemeinschaft, Kennedyallee 40, 53175 Bonn, Deutschland
Abstract
The working group “Analyses in Biological Materials” of the Permanent Senate Commission for the Investigation of Health Hazards of Chemical Compounds in the Work Area validated the presented biomonitoring method.
Mercury is determined by flow injection cold vapour atomic absorption spectrometry (CV‐AAS). The digested blood or urine samples are stabilised with potassium permanganate, introduced into the acid carrier flow (hydrochloric acid) and mixed with the reducing agent sodium borohydride. Mercury vapour formed by reduction is transported with an argon flow into the atomisation cell of the AA spectrometer.
Calibration is performed using matrix matched calibration solutions. The mercury concentrations in real samples are calculated from the linear relationship between the measured absorbance and the mass concentration of mercury.
