Boric acid and tetraborates – Determination of boron in urine by ICP‐OES
Biomonitoring Method – Translation of the German version from 2019
Bernhard Michalke1Peter Grill1
Jackie Morton2
Thomas Göen3
Andrea Hartwig4
MAK Commission5
1 Helmholtz Zentrum München, German Research Center for Environmental Health (GmbH), Research Unit Analytical BioGeoChemistry, Ingolstädter Landstraße 1, 85764 Neuherberg, Germany
2 Health & Safety Laboratory, Harpur Hill, SK17 9JN Buxton, Vereinigtes Königreich
3 Friedrich-Alexander-Universität Erlangen-Nürnberg, Institute and Outpatient Clinic of Occupational, Social, and Environmental Medicine, Henkestraße 9–11, 91054 Erlangen, Germany
4 Institute of Applied Biosciences, Department of Food Chemistry and Toxicology, Karlsruhe Institute of Technology (KIT), Adenauerring 20a, Building 50.41, 76131 Karlsruhe, Germany
5 Permanent Senate Commission for the Investigation of Health Hazards of Chemical Compounds in the Work Area, Deutsche Forschungsgemeinschaft, Kennedyallee 40, 53175 Bonn, Germany
Abstract
The working group “Analyses in Biological Materials” of the Permanent Senate Commission for the Investigation of Health Hazards of Chemical Compounds in the Work Area developed and validated the presented biomonitoring method.
The analytical method described hereinafter is used to determine the boron concentration in urine using inductively coupled plasma optical emission spectrometry (ICP‐OES). The method is rapid, simple, reliable, adequately sensitive and also suitable for routine use in laboratories with high sample throughput. It is possible to determine boron at both occupational and environmental concentrations. Sample preparation is performed by 1/20 (V/V) dilution of urine with 5% nitric acid, which largely reduces matrix interferences.
