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    9. 5‐Fluoruracil – Bestimmung von α‐Fluor‐β‐alanin in Urin mittels GC‐MS/MS. Biomonitoring-Methode
Cover: The MAK Collection for Occupational Health and Safety

The MAK Collection for Occupational Health and Safety

German Research Foundation – Permanent Senate Commission for the Investigation of Health Hazards of Chemical Compounds in the Work Area
(MAK Commission)

ISSN 2509-2383



5‐Fluoruracil – Bestimmung von α‐Fluor‐β‐alanin in Urin mittels GC‐MS/MS

Biomonitoring-Methode

Rudolf Schierl1
Elke Fischer1
Gerhard Scherer2
Heinz Werner Hagedorn2
  Thomas Göen3
  Andrea Hartwig4
  MAK Commission5

1 Institut und Poliklinik für Arbeits‐, Sozial‐ und Umweltmedizin, Ziemssenstraße 1, 80336 München, Deutschland
2 ABF Analytisch‐Biologisches Forschungslabor GmbH, Semmelweisstraße 5, 82152 Planegg, Deutschland
3 Friedrich-Alexander-Universität Erlangen-Nürnberg, Institut und Poliklinik für Arbeits-, Sozial- und Umweltmedizin, Henkestraße 9–11, 91054 Erlangen, Deutschland
4 Institut für Angewandte Biowissenschaften, Abteilung Lebensmittelchemie und Toxikologie, Karlsruher Institut für Technologie (KIT), Adenauerring 20a, Geb. 50.41, 76131 Karlsruhe, Deutschland
5 Ständige Senatskommission zur Prüfung gesundheitsschädlicher Arbeitsstoffe, Deutsche Forschungsgemeinschaft, Kennedyallee 40, 53175 Bonn, Deutschland

Abstract

The working group “Analyses in Biological Materials” of the Permanent Senate Commission for the Investigation of Health Hazards of Chemical Compounds in the Work Area verified the presented biomonitoring method.

The method described hereinafter permits the determination of α‐fluoro‐β‐alanine (FBAL), the main metabolite of 5‐fluorouracil, at low concentration levels and can be used for the biomonitoring of exposed individuals (manufacture, pharmacy, medicine).

To this end, urine samples are spiked with 13C‐FBAL as internal standard (IS) and acidified with 0.1 M hydrochloric acid. The samples are then extracted and purified using a strong cation exchanger. After resuspension and derivatisation, FBAL is analysed using GC‐MS/MS in the MRM (Multiple Reaction Monitoring) mode and quantified with reference to the internal standard. Calibration standards are prepared in pooled urine and processed in the same way as the samples to be analysed.

Keywords

5‐fluorouracil, α‐fluoro‐β‐alanine, biomonitoring, urine, GC‐MS/MS