5‐Fluorouracil – Determination of α‐fluoro‐β‐alanine in urine by GC‐MS/MS
Biomonitoring Method – Translation of the German version from 2019
Rudolf Schierl1Elke Fischer1
Gerhard Scherer2
Heinz Werner Hagedorn2
Thomas Göen3
Andrea Hartwig4
MAK Commission5
1 Institute and Outpatient Clinic for Occupational, Social, and Environmental Medicine, Ziemssenstraße 1, 80336 München, Germany
2 ABF Analytisch‐Biologisches Forschungslabor GmbH, Semmelweisstraße 5, 82152 Planegg, Germany
3 Friedrich-Alexander-Universität Erlangen-Nürnberg, Institute and Outpatient Clinic of Occupational, Social, and Environmental Medicine, Henkestraße 9–11, 91054 Erlangen, Germany
4 Institute of Applied Biosciences, Department of Food Chemistry and Toxicology, Karlsruhe Institute of Technology (KIT), Adenauerring 20a, Building 50.41, 76131 Karlsruhe, Germany
5 Permanent Senate Commission for the Investigation of Health Hazards of Chemical Compounds in the Work Area, Deutsche Forschungsgemeinschaft, Kennedyallee 40, 53175 Bonn, Germany
Abstract
The working group “Analyses in Biological Materials” of the Permanent Senate Commission for the Investigation of Health Hazards of Chemical Compounds in the Work Area verified the presented biomonitoring method.
The method described hereinafter permits the determination of
To this end, urine samples are spiked with 13C‐FBAL as internal standard (IS) and acidified with 0.1 M hydrochloric acid. The samples are then extracted and purified using a strong cation exchanger. After resuspension and derivatisation, FBAL is analysed using GC‐MS/MS in the MRM (Multiple Reaction Monitoring) mode and quantified with reference to the internal standard. Calibration standards are prepared in pooled urine and processed in the same way as the samples to be analysed.
