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    9. 4‐tert‐Octylphenol and p‐nonylphenol – Determination of 4‐tert‐octylphenol and p‐nonylphenol in urine by LC‐MS/MS. Biomonitoring Method – Translation of the German version from 2019
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The MAK Collection for Occupational Health and Safety

Deutsche Forschungsgemeinschaft – Ständige Senatskommission zur Prüfung gesundheitsschädlicher Arbeitsstoffe (MAK-Kommission)

ISSN 2509-2383



4‐tert‐Octylphenol and p‐nonylphenol – Determination of 4‐tert‐octylphenol and p‐nonylphenol in urine by LC‐MS/MS

Biomonitoring Method – Translation of the German version from 2019

Wolfgang Gries1
Gabriele Leng2
Katja Küpper2
Katharina Blümlein3
Susanne Gerling3
  Thomas Göen4
  Andrea Hartwig5
  MAK Commission6

1 Currenta GmbH & Co. OHG, CUR‐SEL‐ANT‐UWA, R800, 12, 47829 Uerdingen, Germany
2 Currenta GmbH & Co. OHG, CUR‐SEL‐SER‐GS‐BLM – Institute for Biomonitoring, Building L9, 51368 Leverkusen, Germany
3 Fraunhofer Institute for Toxicology and Experimental Medicine (ITEM), Nikolai‐Fuchs‐Straße 1, 30625 Hannover, Germany
4 Friedrich-Alexander-Universität Erlangen-Nürnberg, Institute and Outpatient Clinic of Occupational, Social, and Environmental Medicine, Henkestraße 9–11, 91054 Erlangen, Germany
5 Institute of Applied Biosciences, Department of Food Chemistry and Toxicology, Karlsruhe Institute of Technology (KIT), Adenauerring 20a, Building 50.41, 76131 Karlsruhe, Germany
6 Permanent Senate Commission for the Investigation of Health Hazards of Chemical Compounds in the Work Area, Deutsche Forschungsgemeinschaft, Kennedyallee 40, 53175 Bonn, Germany

Abstract

The working group “Analyses in Biological Materials” of the Permanent Senate Commission for the Investigation of Health Hazards of Chemical Compounds in the Work Area developed and validated the presented biomonitoring method. The analytical method described hereinafter permits the selective detection of 4‐tert-octylphenol as well as the sum of various branched p‐nonylphenol isomers in urine. After adding the labelled internal standards (13C6‐4‐tert-octylphenol and 13C6-p‐nonylphenol), the samples are enzymatically hydrolysed to release the analytes from the conjugated alkylphenols. After online SPE, the analytes are separated by liquid chromatography and analysed using tandem mass spectrometry. A quantitation limit of 2 µg/l each is obtained for the analytes. Calibration standards are prepared in pooled urine and processed in the same way as the samples to be analysed.

Keywords

Alkylphenole, 4‐tert‐Octylphenol, p‐Nonylphenol, Biomonitoring, Urin, LC‐MS/MS