1‐Bromopropane and 2‐bromopropane – Determination of 1‐bromopropane and 2‐bromopropane in urine by dynamic headspace GC/MS
Biomonitoring Method – Translation of the German version from 2019
Bernd Roßbach1Eleonore Rißler1
Lygia Therese Budnik2
Susanne Finger2
Thomas Göen3
Andrea Hartwig4
MAK Commission5
1 Institute for Occupational, Social and Environmental Medicine, Obere Zahlbacher Straße 67, 55131 Mainz, Germany
2 Institute for Occupational and Maritime Medicine, Marckmannstraße 129 b, 20539 Hamburg, Germany
3 Friedrich-Alexander-Universität Erlangen-Nürnberg, Institute and Outpatient Clinic of Occupational, Social, and Environmental Medicine, Henkestraße 9–11, 91054 Erlangen, Germany
4 Institute of Applied Biosciences, Department of Food Chemistry and Toxicology, Karlsruhe Institute of Technology (KIT), Adenauerring 20a, Building 50.41, 76131 Karlsruhe, Germany
5 Permanent Senate Commission for the Investigation of Health Hazards of Chemical Compounds in the Work Area, Deutsche Forschungsgemeinschaft, Kennedyallee 40, 53175 Bonn, Germany
Abstract
The working group “Analyses in Biological Materials” of the Permanent Senate Commission for the Investigation of Health Hazards of Chemical Compounds in the Work Area verified the presented biomonitoring method.
The analytical method described herein permits the sensitive determination of both unmetabolised 1‐bromopropane and 2‐bromopropane in urine. Prior to the determination using GC‐MS in the Single‐Ion‐Mode (SIM), the analytes are extracted and enriched using Headspace Solid Phase Dynamic Extraction (SPDE). To this end, the urine samples are incubated at 50 °C and the analytes are extracted from the gas phase using SPDE. The headspace extract is then injected into the gas chomatograph for separation and mass spectrometric analysis. Calibration standards are prepared in urine and processed in the same way as the samples to be analysed. Deuterated benzene is used as the internal standard (IS).
