Formamide, dimethylformamide – Determination of formamide in urine by gas chromatography mass spectrometry
Biomonitoring Method – Translation of the German version from 2018
Gerhard Scherer1 (Method development)Gerhard Gilch1 (Method development)
Beate Aust2 (External verification)
Meinolf Blaszkewicz2 (External verification)
Thomas Göen3 (Head of the working group “Analyses in Biological Materials” of the Permanent Senate Commission for the Investigation of Health Hazards of Chemical Compounds in the Work Area, Deutsche Forschungsgemeinschaft)
Andrea Hartwig4 (Chair of the Permanent Senate Commission for the Investigation of Health Hazards of Chemical Compounds in the Work Area, Deutsche Forschungsgemeinschaft)
MAK Commission5
1 ABF Analytisch‐biologisches Forschungslabor GmbH, Goethestraße 20, 80336 München, Germany
2 Leibniz‐Institut für Arbeitsforschung an der TU Dortmund, Ardeystraße 67, 44139 Dortmund, Germany
3 Friedrich-Alexander-Universität Erlangen-Nürnberg, Institute and Outpatient Clinic of Occupational, Social, and Environmental Medicine, Schillerstraße 25 and 29, 91054 Erlangen, Germany
4 Institute of Applied Biosciences, Department of Food Chemistry and Toxicology, Karlsruhe Institute of Technology (KIT), Adenauerring 20a, Building 50.41, 76131 Karlsruhe, Germany
5 Permanent Senate Commission for the Investigation of Health Hazards of Chemical Compounds in the Work Area, Deutsche Forschungsgemeinschaft, Kennedyallee 40, 53175 Bonn, Germany
Abstract
The working group “Analyses in Biological Materials” of the Permanent Senate Commission for the Investigation of Health Hazards of Chemical Compounds in the Work Area verified the presented biomonitoring method.
The method described herein allows the determination of formamide in urine by gas chromatography mass spectrometry (GC‐MS). Due to its sensitivity, this method is suitable for the detection of occupational and environmental exposure to formamide. For the analytical determination 1 ml of urine is lyophilised after being spiked with 13C,15N‐formamide as the internal standard. The lyophilisate is extracted with 200 µl methanol. After centrifugation, 1 µl of the extract is injected into a GC‐MS system. The method was extensively validated and the reliability data were confirmed by an independent laboratory, which has established and cross‐checked the whole procedure.
